Journal: Journal of Medical Virology

Article Title: SARS‐CoV‐2 pseudovirus infectivity and expression of viral entry‐related factors ACE2, TMPRSS2, Kim‐1, and NRP‐1 in human cells from the respiratory, urinary, digestive, reproductive, and immune systems

doi: 10.1002/jmv.27244

Figure Lengend Snippet: Cell lines used in the present study

Article Snippet: BC‐3 , Primary effusion lymphoma , ATCC CRL‐2277 , RPMI‐1640 complete medium.

Techniques: Cell Culture

Journal: Scientific reports

Article Title: Co-aggregation of MSC/chondrocyte in a dynamic 3D culture elevates the therapeutic effect of secreted extracellular vesicles on osteoarthritis in a rat model.

doi: 10.1038/s41598-022-22592-4

Figure Lengend Snippet: Figure 2. qRT-PCR analysis of chondrogenic genes. (A) Histogram displays the qRT-PCR analysis of collagen type II (Col II), Sox9, aggrecan (Acan), and Col X in a cell micromass culture in the existence of extracellular vesicles (EVs) derived from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days in vitro histological analysis. (B, a) Statistical analysis of the comparison between micromass diameters of all experimental groups and the control that demonstrated the Co-ag-EV + and MSC-ag-EV + groups show bigger size and differentiation phenotype of MSCs to chondrocytes than the control group (received chondrogenic medium contain TGFß1) and Cho-ag-EV group. (B, b) Toluidine blue was performed for (TB) of mesenchymal stem cells (MSCs) micromass sections that differentiated into chondrocytes in the presence of extracellular vesicles (EVs) harvested from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days. (B, c) Statistical analysis of TB staining based on the percent of positive staining area. TB staining does not display a statistically significant difference between the groups. (B, d) safranin O (SO) staining was performed for all micromass sections. (B, e) Statistical analysis of SO staining based on the percent of positive staining area. SO staining does not display a statistically significant difference between the groups. The results are expressed as the mean ± SEM. *Significant difference compared with the control group (*p < 0.05; **p < 0.01).

Article Snippet: To assess the chondrogenic proteins expression level in the extracted EVs, we also executed a wet western blot analysis with the primary antibodies of COL II (COL2A1, 250,484, Abbiotec, USA), aggrecan (ACAN, NB600-504, Novus Biologicals, USA), and COL X (COL10A, ab58632, Abcam, UK).

Techniques: Quantitative RT-PCR, Derivative Assay, In Vitro, Comparison, Control, Staining

Journal: Microorganisms

Article Title: Cultivable Bacterial Communities in Brines from Perennially Ice-Covered and Pristine Antarctic Lakes: Ecological and Biotechnological Implications

doi: 10.3390/microorganisms8060819

Figure Lengend Snippet: Boulder Clay and Tarn Flat viable counts (CFU mL −1 × 10 3 ) and number of isolates (in brackets) obtained from direct plating per sample and isolation medium.

Article Snippet: BC3 showed growth on DSMZ 97 medium (0.1 × 10 3 CFU mL −1 ) but no colonies were yielded.

Techniques: Isolation

Journal: Microorganisms

Article Title: Cultivable Bacterial Communities in Brines from Perennially Ice-Covered and Pristine Antarctic Lakes: Ecological and Biotechnological Implications

doi: 10.3390/microorganisms8060819

Figure Lengend Snippet: 16S rRNA gene sequence affiliation to their closet phylogenetic neighbours of bacterial isolates from Antarctic lake brines. Numbers in brackets indicate the number of isolates per medium. Unidentified isolates are not listed.

Article Snippet: BC3 showed growth on DSMZ 97 medium (0.1 × 10 3 CFU mL −1 ) but no colonies were yielded.

Techniques: Sequencing, Isolation